1SMY
Structural basis for transcription regulation by alarmone ppGpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45PX |
Synchrotron site | SPring-8 |
Beamline | BL45PX |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-15 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 32 |
Unit cell lengths | 236.350, 236.350, 249.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.700 |
R-factor | 0.266 |
Rwork | 0.186 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw7 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.080 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.900 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 0.430 |
Number of reflections | 409145 | |
<I/σ(I)> | 3.1 | |
Completeness [%] | 95.8 | 92.4 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | MG FORMATE, PEG400, SPERMIDINE, TRIS HCL, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 293K |