1SME

PLASMEPSIN II, A HEMOGLOBIN-DEGRADING ENZYME FROM PLASMODIUM FALCIPARUM, IN COMPLEX WITH PEPSTATIN A

Experimental procedure

Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	142.100, 142.100, 97.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.198 *
Rwork	0.195
RMSD bond length	0.011
RMSD bond angle	26.900 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.940
Total number of observations	63000 *
Number of reflections	22465 *
Completeness [%]	77.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	phosphate	0.1 (M)
3	1	reservoir		47 (%)