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1SI2

Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex of deoxynucleotide overhang

Experimental procedure
Experimental methodSAD
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-ID-B
Synchrotron siteAPS
Beamline14-ID-B
Temperature [K]100
Detector technologyCCD
Collection date2003-11-13
DetectorMARRESEARCH
Wavelength(s)0.9810
Spacegroup nameP 64
Unit cell lengths100.051, 100.051, 34.438
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution19.710 - 2.600
R-factor0.2247
Rwork0.221
R-free0.26100
Structure solution methodSAD
RMSD bond length0.007
RMSD bond angle1.200
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS (1.1)
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.690
High resolution limit [Å]2.6002.600
Number of reflections6225
<I/σ(I)>27.83.1
Completeness [%]99.0100
Redundancy10.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.6293PEG 1000, potassium chloride, HEPES-NaOH, Dithiothreitol, pH 7.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111PEG 1000
211potassium chloride
311HEPES-NaOH
411Dithiothreitol

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