1SGU
Comparing the Accumulation of Active Site and Non-active Site Mutations in the HIV-1 Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 |
| Unit cell lengths | 61.573, 61.574, 84.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.600 - 1.900 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 1.261 |
| RMSD bond angle | 3.300 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14298 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 96.6 | 80.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 20 mM Sodium acetate, 1.5 M ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K, pH 4.50 |
