1SB7
Crystal structure of the E.coli pseudouridine synthase TruD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-18 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.665, 108.780, 111.855 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.690 - 2.200 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25400 |
Structure solution method | SIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.690 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.414 |
Number of reflections | 39254 | |
<I/σ(I)> | 43.1 | 7.2 |
Completeness [%] | 97.8 | 99.6 |
Redundancy | 10.76 | 10.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | magnesium chloride, Hepes 7.5, isopropanol, PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |