1SAX
Three-dimensional structure of s.aureus methicillin-resistance regulating transcriptional repressor meci in complex with 25-bp ds-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.600, 133.600, 54.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.700 - 2.800 |
| R-factor | 0.21624 |
| Rwork | 0.212 |
| R-free | 0.27601 |
| Starting model (for MR) | 1okr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.938 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.500 |
| Number of reflections | 13777 | |
| <I/σ(I)> | 19.7 | 2.3 |
| Completeness [%] | 97.8 | 93.6 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 8000, magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 2 | PEG 8000 | ||
| 4 | 1 | 2 | MgCl2 |
