Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.462, 111.462, 74.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.800 |
| R-factor | 0.18397 |
| Rwork | 0.182 |
| R-free | 0.22148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RRG and coordinates of arf1[D1-17]/Gea2-Sec7 (Goldberg J. (1998) Cell 95(2): 237-48) |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.940 |
| Data scaling software | XDS |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 43814 | |
| <I/σ(I)> | 26.2 | 6.4 |
| Completeness [%] | 98.1 | 97.1 |
| Redundancy | 12 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 29% PEG 3350, 40MM NH4CL, 40MM POTASSIUM FORMATE, 100MM TRIS, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
