Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.980 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.462, 111.462, 74.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.880 - 1.800 |
R-factor | 0.18397 |
Rwork | 0.182 |
R-free | 0.22148 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RRG and coordinates of arf1[D1-17]/Gea2-Sec7 (Goldberg J. (1998) Cell 95(2): 237-48) |
RMSD bond length | 0.025 |
RMSD bond angle | 1.940 |
Data scaling software | XDS |
Phasing software | CCP4 ((MOLREP)) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 43814 | |
<I/σ(I)> | 26.2 | 6.4 |
Completeness [%] | 98.1 | 97.1 |
Redundancy | 12 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 29% PEG 3350, 40MM NH4CL, 40MM POTASSIUM FORMATE, 100MM TRIS, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |