1S95
Structure of serine/threonine protein phosphatase 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.808, 80.309, 92.189 |
| Unit cell angles | 90.00, 94.25, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| R-factor | 0.17125 |
| Rwork | 0.169 |
| R-free | 0.20933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partial homology model derived from 1IT6 1auj 1g5b |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.553 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.590 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.064 | |
| Number of reflections | 70964 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 70.0 | 13 |
| Redundancy | 2.9 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2-methyl,2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
