1S95
Structure of serine/threonine protein phosphatase 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.808, 80.309, 92.189 |
Unit cell angles | 90.00, 94.25, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.600 |
R-factor | 0.17125 |
Rwork | 0.169 |
R-free | 0.20933 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Partial homology model derived from 1IT6 1auj 1g5b |
RMSD bond length | 0.021 |
RMSD bond angle | 1.553 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.590 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.064 | |
Number of reflections | 70964 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 70.0 | 13 |
Redundancy | 2.9 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2-methyl,2,4-pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |