1S64

Rat protein geranylgeranyltransferase type-I complexed with L-778,123 and a sulfate anion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-19
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	271.153, 268.681, 184.622
Unit cell angles	90.00, 131.55, 90.00

Refinement procedure

Resolution	30.000 - 2.550
R-factor	0.192
Rwork	0.192
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n4q
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	300151
<I/σ(I)>	15.2	3.1
Completeness [%]	93.3	85.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.3	290	1.3 M ammonium sulfate, 175 mM NaCitrate pH 6.5, 100 mM MES pH 6.3, 20 mM DTT, 1uM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 6.30