1S64
Rat protein geranylgeranyltransferase type-I complexed with L-778,123 and a sulfate anion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-19 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 271.153, 268.681, 184.622 |
Unit cell angles | 90.00, 131.55, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.550 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n4q |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 300151 | |
<I/σ(I)> | 15.2 | 3.1 |
Completeness [%] | 93.3 | 85.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.3 | 290 | 1.3 M ammonium sulfate, 175 mM NaCitrate pH 6.5, 100 mM MES pH 6.3, 20 mM DTT, 1uM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 6.30 |