1RZF
Crystal structure of Human anti-HIV-1 GP120-reactive antibody E51
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.770, 71.608, 99.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.1981 |
Rwork | 0.198 |
R-free | 0.23300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rz7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.365 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.067 * | |
Total number of observations | 757387 * | |
Number of reflections | 60893 | |
Completeness [%] | 98.8 * | 89.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | 14% PEG 4000, 7% isopropanol, 0.07M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 2.5 (mM) | |
2 | 1 | drop | 0.35 (M) | ||
3 | 1 | drop | 0.02 (%(v/v)) | pH7.0 | |
4 | 1 | drop | protein | 4-8 (mg/ml) | |
5 | 1 | reservoir | PEG4000 | 14 (%) | |
6 | 1 | reservoir | isopropyl alcohol | 7 (%) | |
7 | 1 | reservoir | HEPES | 0.07 (M) | pH7.5 |