1RY7
Crystal Structure of the 3 Ig form of FGFR3c in complex with FGF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-07-03 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.223, 64.748, 99.932 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| R-factor | 0.2771 |
| Rwork | 0.277 |
| R-free | 0.34100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FGF1-FGFR1c complex (pdb id code 1EVT) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.068 * | 0.303 * |
| Total number of observations | 41929 * | |
| Number of reflections | 11404 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 98.9 | 95.5 |
| Redundancy | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | Peg 4000, glycerol, MPD, cadmium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 16 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | glycerol | 5 (%) | |
| 4 | 1 | reservoir | MPD | 5 (%) | |
| 5 | 1 | reservoir | 25000 (nM) | ||
| 6 | 1 | drop | HEPES | 25 (mM) | pH7.5 |
| 7 | 1 | drop | 150 (mM) | ||
| 8 | 1 | drop | protein | 14 (mg/ml) |
