1RE0
Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.725, 72.294, 119.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.400 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.23800 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.061 | 0.249 * |
Total number of observations | 136824 * | |
Number of reflections | 22531 | 2221 * |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 400, sodium citrate, Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium citrate | 50 (mM) | |
2 | 1 | reservoir | PEG400 | 19 (%(w/v)) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
4 | 1 | drop | HEPES | 20 (mM) | |
5 | 1 | drop | 150 (mM) | ||
6 | 1 | drop | dithiothreitol | 4 (mM) |