1R9T
RNA POLYMERASE II STRAND SEPARATED ELONGATION COMPLEX, MISMATCHED NUCLEOTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-21 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.846, 222.958, 193.602 |
| Unit cell angles | 90.00, 101.17, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.500 |
| R-factor | 0.239 |
| Rwork | 0.230 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i6h |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.370 | |
| Number of reflections | 83860 | |
| Completeness [%] | 94.2 | 87.3 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 6000, Ammonium hydrogen phosphate, sodium dihydrogen phosphate, dioxane, DTT, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Ammonium hydrogen phosphate | ||
| 3 | 1 | 1 | sodium dihydrogen phosphate | ||
| 4 | 1 | 1 | dioxane | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 6000 | ||
| 8 | 1 | 2 | Ammonium hydrogen phosphate | ||
| 9 | 1 | 2 | sodium dihydrogen phosphate |
