1R6T
crystal structure of human tryptophanyl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97941 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.146, 95.713, 98.506 |
| Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.2082 |
| Rwork | 0.208 |
| R-free | 0.23860 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.099 | 0.614 * |
| Number of reflections | 82479 * | |
| <I/σ(I)> | 13.87 | |
| Completeness [%] | 96.5 * | 87.3 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 4 * | PEG 8K, Mes, pH 6.32, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 2 (%) | |
| 2 | 1 | reservoir | sodium-MES | 0.1 (M) | pH6.32 |
| 3 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 0.02 (%) | ||
| 6 | 1 | drop | 2-mercaptoethanol | 2 (mM) |
