1R15
Aplysia ADP ribosyl cyclase with bound nicotinamide and R5P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 200 |
Wavelength(s) | 0.919 |
Spacegroup name | P 1 |
Unit cell lengths | 60.400, 75.320, 138.130 |
Unit cell angles | 88.16, 89.22, 89.09 |
Refinement procedure
Resolution | 33.000 - 2.400 |
R-factor | 0.245 |
Rwork | 0.244 |
R-free | 0.26940 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.692 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | |
High resolution limit [Å] | 2.400 | 2.400 * |
Rmerge | 0.108 * | 0.310 * |
Number of reflections | 57197 | |
Completeness [%] | 88.0 | 44.7 * |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | Munshi, C., (1999) J. Biol. Chem., 274, 30770. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 17 (%(w/v)) | |
2 | 1 | reservoir | imidazole | 0.1 (M) | pH7.5 |