1R0K
Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8452 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.700, 93.200, 98.600 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 29.630 - 1.910 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jvs 1-deoxy-D-xylulose 5-phosphate reductoisomerase (E.coli) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.630 | 1.990 |
| High resolution limit [Å] | 1.910 | 1.900 |
| Rmerge | 0.420 | |
| Number of reflections | 118460 | |
| <I/σ(I)> | 9.5 | 2.5 |
| Completeness [%] | 97.9 | 95.1 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Na citrate, PEG 4000, Ammonium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
