1QZY

Human Methionine Aminopeptidase in complex with bengamide inhibitor LAF153 and cobalt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	C 2 2 21
Unit cell lengths	90.610, 99.040, 101.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 1.600
R-factor	0.204
Rwork	0.204
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.400
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.060 *	0.450 *
Total number of observations	679600 *
Number of reflections	59626 *
Completeness [%]	98.8 *	89.8 *
Redundancy	11.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH7.0
2	1	drop		150 (mM)
3	1	drop		1 (mM)
4	1	drop	glycerol	10 (%)
5	1	reservoir	t-butyl alcohol	30 (%)
6	1	reservoir	sodium citrate	50 (mM)	pH5.5