1QHF

YEAST PHOSPHOGLYCERATE MUTASE-3PG COMPLEX STRUCTURE TO 1.7 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	96.400, 85.900, 81.900
Unit cell angles	90.00, 120.60, 90.00

Refinement procedure

Resolution	20.000 - 1.700
Rwork	0.177
R-free	0.21500
Structure solution method	OTHER
Starting model (for MR)	3pgm
RMSD bond length	0.012
RMSD bond angle	0.029
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.300
High resolution limit [Å]	1.700	1.700
Rmerge	0.088 *
Number of reflections	42944
Completeness [%]	82.5	77.2
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8	277 *	55% AMMONIUM SULFATE IN 10MM IMIDAZOLE BUFFER PH 6.8 WITH 1MM 3PG AND CONCENTRATION OF 10 MG ML PROTEIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	55 (%)
3	1	reservoir	imidazole	10 (mM)
4	1	reservoir	3PG substrate	1 (mM)