1QFW

TERNARY COMPLEX OF HUMAN CHORIONIC GONADOTROPIN WITH FV ANTI ALPHA SUBUNIT AND FV ANTI BETA SUBUNIT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	297
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	104.900, 104.900, 150.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.000 - 3.500
R-factor	0.268 *
Rwork	0.260
R-free	0.31600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hrp 1igc 2imn
RMSD bond length	0.016
RMSD bond angle	29.140 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000 *	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.166 *	0.476 *
Number of reflections	12096
<I/σ(I)>	9	3
Completeness [%]	96.6	97
Redundancy	4. *	2.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	20 *	1.8 M ammonium Sulfate, 100 mM tris/HCl pH 8.0, protein concentration 3 mg/ml

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8 (M)
3	1	reservoir	Tris-HCl	100 (mM)	pH8.0