1Q5H
Human dUTP Pyrophosphatase complex with dUDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 73.197, 110.766, 53.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.17877 |
Rwork | 0.177 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dup |
RMSD bond length | 0.012 * |
RMSD bond angle | 2.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.077 | 0.323 |
Total number of observations | 102902 * | |
Number of reflections | 28406 | |
<I/σ(I)> | 10.8 | 4.4 |
Completeness [%] | 94.0 | 82 |
Redundancy | 3.62 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | 1.1M sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | reservoir | sodium citrate | 1.0-1.2 (M) | pH8.0 |
5 | 1 | reservoir | imidazole/malate | 100 (mM) | pH7.5-8.5 |