1Q3V
Crystal structure of a wild-type Cre recombinase-loxP synapse: phosphotyrosine covalent intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 108.938, 164.160, 194.759 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.920 - 2.900 * |
R-factor | 0.2471 |
Rwork | 0.247 |
R-free | 0.26200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1crx |
RMSD bond length | 0.020 * |
RMSD bond angle | 20.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.900 * | 2.910 |
Rmerge | 0.067 * | 0.330 * |
Completeness [%] | 96.2 | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.6 | 293 | NaCl, EDTA, Glycerol, HEPES, MgCl2, PEG 2000, MME, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MME | ||
10 | 1 | 2 | EDTA | ||
11 | 1 | 2 | Glycerol | ||
12 | 1 | 2 | PEG 2000 | ||
2 | 1 | 1 | HEPES | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | EDTA | ||
6 | 1 | 1 | Glycerol | ||
7 | 1 | 1 | PEG 2000 | ||
8 | 1 | 2 | NaCl | ||
9 | 1 | 2 | MgCl2 |