1PX5
Crystal structure of the 2'-specific and double-stranded RNA-activated interferon-induced antiviral protein 2'-5'-oligoadenylate synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.524, 132.601, 57.305 |
Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.740 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22420 * |
Structure solution method | SIRAS |
RMSD bond length | 0.004 |
RMSD bond angle | 1.180 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 1.800 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.042 | 0.219 * |
Number of reflections | 69379 | |
<I/σ(I)> | 32.4 | 8.1 |
Completeness [%] | 99.4 * | 96.7 |
Redundancy | 6.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 20 * | PEG 2000mme, sodium cacodylate, ammonium sulfate, sodium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | PEG2000 MME | 30 (%) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6. |