1PV8

Crystal structure of a low activity F12L mutant of human porphobilinogen synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-08
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	89.571, 89.571, 153.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 2.200
R-factor	0.199
Rwork	0.199
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e51
RMSD bond length	0.018
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.200
Number of reflections	33615 *
Completeness [%]	95.7	72.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	296	50mM BTP, 10mM beta-mercaptoethanol, 10uM ZnCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.0 (mg/ml)
2	1	reservoir	monoammonium hydrogen phosphate	0.4 (M)