1PJA
The crystal structure of palmitoyl protein thioesterase-2 reveals the basis for divergent substrate specificities of the two lysosomal thioesterases (PPT1 and PPT2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.943 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 148.520, 148.520, 152.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.251 |
| Rwork | 0.221 |
| R-free | 0.24500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.090 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.046 * | |
| Number of reflections | 34721 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 100.0 * | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 4 * | 1.8-2.2M ammonium sulfate, 8% methyl-pentane-diol, 100 mM MES , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH5.5-6.5 |
| 4 | 1 | reservoir | MPD | 8 (%) |
