1P9M
Crystal structure of the hexameric human IL-6/IL-6 alpha receptor/gp130 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-09-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 279.800, 279.800, 96.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 3.650 |
| R-factor | 0.282 |
| Rwork | 0.282 |
| R-free | 0.33400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1alu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.840 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.065 | 0.551 |
| Total number of observations | 120957 * | |
| Number of reflections | 16144 * | |
| <I/σ(I)> | 13 | 1.85 |
| Completeness [%] | 98.3 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | Sodium formate, Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 2.0 (M) | pH4.5 |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
