1P6O
The crystal structure of yeast cytosine deaminase bound to 4(R)-hydroxyl-3,4-dihydropyrimidine at 1.14 angstroms.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.880, 70.417, 71.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.140 |
R-factor | 0.1097 |
Rwork | 0.110 |
R-free | 0.15210 |
Structure solution method | SAD |
Starting model (for MR) | 1ox7 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.029 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.180 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.048 | 0.283 |
Number of reflections | 92236 | |
<I/σ(I)> | 20.84 | 3.81 |
Completeness [%] | 92.7 | 61.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microseeding | 6.5 | 277 | PEG 8000, calcium acetate, sodium cacodylate, pH 6.5, microseeding, temperature 277K |