1P22
Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity on the SCFbeta-TrCP1 ubiquitin ligase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2001-12-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9500 |
| Spacegroup name | P 31 |
| Unit cell lengths | 82.600, 82.600, 111.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.23315 |
| Rwork | 0.230 |
| R-free | 0.28600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.855 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.025 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.040 * | 0.430 * |
| Total number of observations | 52111 * | |
| Number of reflections | 17628 | |
| Completeness [%] | 99.3 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | PEG4000, sodium citrate, Bis-Tris Propane, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | beta-catenin peptide | 5 (mg/ml) | |
| 3 | 1 | reservoir | PEG4000 | 16-20 (%) | |
| 4 | 1 | reservoir | sodium citrate | 320 (mM) | |
| 5 | 1 | reservoir | BTP | 100 (mM) | pH6.8 |
