1P1W
Crystal structure of the GluR2 ligand-binding core (S1S2J) with the L483Y and L650T mutations and in complex with AMPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-06-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.973 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.883, 121.471, 47.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 1.800 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.220 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.283 |
Number of reflections | 52581 * | |
<I/σ(I)> | 16.5 | |
Completeness [%] | 96.1 | 90.7 |
Redundancy | 3.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | PEG4000, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | AMPA | 20 (mM) | |
2 | 1 | reservoir | PEG4000 | 14-18 (%) | |
3 | 1 | reservoir | ammonium sulfate | 0.2-0.4 (M) |