1OSF
Human Hsp90 in complex with 17-desmethoxy-17-N,N-Dimethylaminoethylamino-Geldanamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.710, 41.680, 55.980 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.750 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | |
| High resolution limit [Å] | 1.750 * | |
| Rmerge | 0.065 * | 0.317 * |
| Total number of observations | 97350 * | |
| Number of reflections | 21560 | |
| Completeness [%] | 99.2 * | 97.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 5 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 17-DMAG | 2.1 (mM) | |
| 2 | 1 | drop | HEPES | 25 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | protein | 24 (mg/ml) | |
| 5 | 1 | reservoir | MPD | 5-10 (%(v/v)) | |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.5 |
