1OPJ
Structural basis for the auto-inhibition of c-Abl tyrosine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.844, 63.507, 64.051 |
| Unit cell angles | 67.91, 79.76, 84.88 |
Refinement procedure
| Resolution | 30.000 * - 1.700* |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iep |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.800 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 * | 0.509 * |
| Total number of observations | 268488 * | |
| Number of reflections | 61592 | |
| <I/σ(I)> | 9.41 | 2.84 |
| Completeness [%] | 92.9 | 75.8 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | 22% PEG 4000, 100 mM MES pH 5.6, 200 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22 (%(w/v)) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH5.6 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 5 (mM) | |
| 7 | 1 | drop | protein | 35 (mg/ml) |
