1OMW

Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and gamma 2 subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	188.200, 72.500, 122.790
Unit cell angles	90.00, 115.20, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.21648
Rwork	0.202
R-free	0.25196
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ABG 1atp 1bak 1dk8
RMSD bond length	0.024
RMSD bond angle	2.035
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.053 *	0.253 *
Number of reflections	38258 *
<I/σ(I)>	15.3	2.7
Completeness [%]	73.6	12.7
Redundancy	2.4	0.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.75	277	Lodowski, D.T., (2003) Acta Crystallogr., D59, 936. *
1	VAPOR DIFFUSION, HANGING DROP	5.75	277	Lodowski, D.T., (2003) Acta Crystallogr., D59, 936. *
1	VAPOR DIFFUSION, HANGING DROP	5.75	277	Lodowski, D.T., (2003) Acta Crystallogr., D59, 936. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH5.25
3	1	reservoir		200 (mM)
4	1	reservoir	inositol-3,4,5-triphosphate	1 (mM)
5	1	reservoir		5 (mM)
6	1	reservoir	PEG3350	6.9-7.8 (%)