1O5M

Structure of FPT bound to the inhibitor SCH66336

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-02
Detector	RIGAKU RAXIS IV
Spacegroup name	P 61
Unit cell lengths	171.110, 171.110, 69.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.174
Rwork	0.174
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.055	0.211 *
Number of reflections	49482
<I/σ(I)>	23.3
Completeness [%]	95.9	71.9
Redundancy	3.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	4 *	Wu, Z., (1999) Protein Eng., 12, 341. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ethanol	23.6 (mM)
2	1	drop	DMSO	50 (mM)
3	1	reservoir		0.1 (M)
4	1	reservoir	sodium acetate	0.1 (M)	pH5.0