1NZI
Crystal Structure of the CUB1-EGF Interaction Domain of Complement Protease C1s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.134, 47.499, 56.679 |
| Unit cell angles | 87.74, 78.04, 75.67 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.2173 |
| Rwork | 0.216 |
| R-free | 0.24600 * |
| Structure solution method | SAD, MR |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.064 * | 0.143 * |
| Total number of observations | 181271 * | |
| Number of reflections | 52518 * | 7588 * |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 94.4 | 93.3 |
| Redundancy | 3.5 * | 2.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 20 * | PEG 4000, magnesium chloride, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.0-7.8 (mg/ml) | |
| 2 | 1 | drop | 145 (mM) | ||
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | TEA-HCl | 50 (mM) | pH7.4 |
| 5 | 1 | reservoir | PEG400 | 30 (%(v/v)) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
