1NVV

Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.100
Spacegroup name	I 4 2 2
Unit cell lengths	184.163, 184.163, 179.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.000 * - 2.180
R-factor	0.208
Rwork	0.208
R-free	0.24100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nvu
RMSD bond length	0.008
RMSD bond angle	21.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.000 *	2.260
High resolution limit [Å]	2.180	2.180
Rmerge	0.103 *	0.352 *
Total number of observations	329000 *
Number of reflections	74722
<I/σ(I)>	13	3.4
Completeness [%]	95.2	81.9
Redundancy	4.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	phosphate	1.2-1.5 (M)
3	1	reservoir	HEPES	100 (mM)	pH7.5