1NVP
HUMAN TFIIA/TBP/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.916, 0.909 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.624, 90.884, 125.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.100 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 25.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.065 | 0.362 |
Total number of observations | 224980 * | |
Number of reflections | 40386 | |
<I/σ(I)> | 25.6 | 4.1 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.6 | 3.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 22 * | PEG6000, lithium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 22 * | PEG6000, lithium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6000 | ||
2 | 1 | 1 | lithium nitrate | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 2 | CaCl2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | dithiothreitol | 1 (mM) | |
11 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
2 | 1 | drop | Bis-Tris | 2 (mM) | pH7.0 |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | EDTA | 0.1 (mM) | |
5 | 1 | drop | dithiothreitol | 0.5 (mM) | |
6 | 1 | drop | 0.01 (%(w/v)) | ||
7 | 1 | reservoir | bis-Tris | 50 (mM) | pH6.5 |
8 | 1 | reservoir | 100 (mM) | ||
9 | 1 | reservoir | 10 (mM) |