1NQL
Structure of the extracellular domain of human epidermal growth factor (EGF) receptor in an inactive (low pH) complex with EGF.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.94 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.170, 103.660, 101.490 |
Unit cell angles | 90.00, 119.27, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.24747 |
Rwork | 0.241 |
R-free | 0.31000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m6b |
RMSD bond length | 0.016 * |
RMSD bond angle | 1.890 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.048 * | 0.345 * |
Total number of observations | 90194 * | |
Number of reflections | 26060 | |
<I/σ(I)> | 12 | 2.2 |
Completeness [%] | 98.0 | 95.4 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 297 | PEG3400,ammonium sulfate, magnesium sulfate, sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 25 (mM) | pH8.0 |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | reservoir | PEG3400 | 12 (%) | |
4 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
5 | 1 | reservoir | 50 (mM) | ||
6 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.0 |