1NP0
Human lysosomal beta-hexosaminidase isoform B in complex with intermediate analogue NAG-thiazoline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.414, 112.414, 397.232 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.760 - 2.500 |
R-factor | 0.19964 |
Rwork | 0.198 |
R-free | 0.22671 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1nou |
RMSD bond length | 0.011 |
RMSD bond angle | 1.557 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 52315 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5 mg/ml HexB [final], 50% saturated ammonium sulfate, 50 mM potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |