1NL0
Crystal structure of human factor IX Gla domain in complex of an inhibitory antibody, 10C12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 103 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.080 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.089, 71.614, 90.867 |
Unit cell angles | 90.00, 119.14, 90.00 |
Refinement procedure
Resolution | 24.660 - 2.200 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model from swissmodel |
RMSD bond length | 0.006 |
RMSD bond angle | 26.200 * |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.660 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.119 |
Total number of observations | 241036 * | |
Number of reflections | 28585 | 1353 * |
<I/σ(I)> | 25.7 | 5.4 |
Completeness [%] | 81.9 | 38.7 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.0 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.3 (M) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | MPD | 2 (%) | |
6 | 1 | drop | 0.2 (M) | ||
7 | 1 | drop | 2 (mM) | ||
8 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |