1NHC
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.012, 84.130, 96.035 |
| Unit cell angles | 114.32, 98.00, 89.75 |
Refinement procedure
| Resolution | 37.000 * - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1czf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.775 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.384 |
| Total number of observations | 430145 * | |
| Number of reflections | 205268 | |
| <I/σ(I)> | 22.2 | 2.1 |
| Completeness [%] | 97.5 | 95.6 |
| Redundancy | 2.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 295 | PEG 4000, ammonium sulfate, nikkel sulfate, sodium citrate, pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.8 (mg/ml) | |
| 2 | 1 | drop | sodium cirtate | 50 (mM) | pH3.0 |
| 3 | 1 | reservoir | PEG4000 | 18-20 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | 0-10 (mM) | ||
| 6 | 1 | reservoir | sodium citrate | 50 (mM) | pH3.0 |
