1NFR

Rv2002 gene product from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9500
Spacegroup name	I 4
Unit cell lengths	120.020, 120.020, 140.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.100
R-factor	0.203
Rwork	0.203
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.075 *	0.271 *
Total number of observations	288652 *
Number of reflections	57609
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	296	ammonium sulfate, PEG 400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)
2	1	reservoir	PEG400	5.0 (%(v/v))
3	1	reservoir	sodium HEPES	100 (mM)	pH7.5
4	1	drop	protein	14.4 (mg/ml)
5	1	drop	Tris-HCl	20 (mM)	pH8.0
6	1	drop	PMSF	0.1 (mM)
7	1	drop	NAD+	2 (mM)