1NFR
Rv2002 gene product from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9500 |
| Spacegroup name | I 4 |
| Unit cell lengths | 120.020, 120.020, 140.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 * | 0.271 * |
| Total number of observations | 288652 * | |
| Number of reflections | 57609 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 296 | ammonium sulfate, PEG 400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 2 | 1 | reservoir | PEG400 | 5.0 (%(v/v)) | |
| 3 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | drop | protein | 14.4 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 6 | 1 | drop | PMSF | 0.1 (mM) | |
| 7 | 1 | drop | NAD+ | 2 (mM) |
