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1N7S

High Resolution Structure of a Truncated Neuronal SNARE Complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.1
Synchrotron siteALS
Beamline8.2.1
Temperature [K]100
Detector technologyCCD
Collection date2002-09-06
DetectorADSC QUANTUM 4
Wavelength(s)0.9000
Spacegroup nameP 21 21 21
Unit cell lengths39.767, 51.750, 132.938
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution34.130 - 1.450
R-factor0.201
Rwork0.198
R-free0.22400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1sfc
RMSD bond length0.006
RMSD bond angle14.300

*

Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS (1.1)
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]34.1401.500
High resolution limit [Å]1.4501.450
Rmerge0.6900.439
Number of reflections485744310

*

<I/σ(I)>20.43.5
Completeness [%]97.888.4
Redundancy55
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.8

*

4 and 20

*

MPD, CaCl2, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 4.0K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein9 (mg/ml)
21drop200 (mM)
31dropHEPES10 (mM)pH7.8
41dropdithiothreitol5 (mM)
51reservoirMPD15-20 (%)
61reservoir75-125 (mM)
71reservoirMES50 (mM)pH5.0-6.0

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PDB entries from 2024-11-20

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