1N7S
High Resolution Structure of a Truncated Neuronal SNARE Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.767, 51.750, 132.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.130 - 1.450 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sfc |
RMSD bond length | 0.006 |
RMSD bond angle | 14.300 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.140 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.690 | 0.439 |
Number of reflections | 48574 | 4310 * |
<I/σ(I)> | 20.4 | 3.5 |
Completeness [%] | 97.8 | 88.4 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 4 and 20 * | MPD, CaCl2, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 4.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | HEPES | 10 (mM) | pH7.8 |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | MPD | 15-20 (%) | |
6 | 1 | reservoir | 75-125 (mM) | ||
7 | 1 | reservoir | MES | 50 (mM) | pH5.0-6.0 |