1N5Y
HIV-1 Reverse Transcriptase Crosslinked to Post-Translocation AZTMP-Terminated DNA (Complex P)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2000-10-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 166.700, 166.700, 221.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.420 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.110 * | |
Total number of observations | 930560 * | |
Number of reflections | 61682 | |
<I/σ(I)> | 1.3 | |
Completeness [%] | 96.7 | 82.7 * |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 40 * | cacodylate, SAS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | cacodylate | ||
2 | 1 | 1 | SAS |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | cacodylate | 100 (mM) | pH5.6 |
3 | 1 | reservoir | ammonium sulfate | 31-34 (%sat) |