1N5Y

HIV-1 Reverse Transcriptase Crosslinked to Post-Translocation AZTMP-Terminated DNA (Complex P)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	105
Detector technology	CCD
Collection date	2000-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 32 1 2
Unit cell lengths	166.700, 166.700, 221.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.100
R-factor	0.255
Rwork	0.255
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.420 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.110 *
Total number of observations	930560 *
Number of reflections	61682
<I/σ(I)>		1.3
Completeness [%]	96.7	82.7 *
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	40 *	cacodylate, SAS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	cacodylate
2	1	1	SAS

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)	pH5.6
3	1	reservoir	ammonium sulfate	31-34 (%sat)