1N0W

Crystal structure of a RAD51-BRCA2 BRC repeat complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.90831
Spacegroup name	P 21 21 21
Unit cell lengths	57.300, 59.140, 77.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.800 * - 1.700
Rwork	0.191
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.229 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.850	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.077	0.321
Total number of observations	204230 *
Number of reflections	29143 *
<I/σ(I)>	23.5	6.5
Completeness [%]	99.9	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	18 *	ETHYLENE GLYCOL, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethylene glycol	25 (%)