1MYW
CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 82.704, 82.704, 72.555 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.880 - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yfp |
| RMSD bond length | 0.001 * |
| RMSD bond angle | 1.376 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.880 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.280 * |
| Rmerge | 0.076 | 0.317 |
| Number of reflections | 121451 * | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 97.7 | 94.5 |
| Redundancy | 8.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 298 | Tris, ammonium sulfate, PEG400, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 33 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.9 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.4 |
| 7 | 1 | reservoir | PEG400 | 4 (%(v/v)) |
