1MW9
Crystal Structure of H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.990, 78.705, 139.328 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.670 |
R-factor | 0.20885 |
Rwork | 0.208 |
R-free | 0.22700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ecl |
RMSD bond length | 0.005 |
RMSD bond angle | 1.170 * |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.670 | |
Rmerge | 0.057 * | 0.407 * |
Total number of observations | 398050 * | |
Number of reflections | 79717 | |
<I/σ(I)> | 8.2 | |
Completeness [%] | 98.4 | 98.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 287 | ammonium sulfate, pH 8, DIALYSIS, temperature 287K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.1 (M) |