1MOX
Crystal Structure of Human Epidermal Growth Factor Receptor (residues 1-501) in complex with TGF-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.590, 198.710, 78.900 |
Unit cell angles | 90.00, 102.03, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.24 |
Rwork | 0.237 |
R-free | 0.28900 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.075 |
<I/σ(I)> | 10.8 |
Completeness [%] | 90.2 * |
Redundancy | 3.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 293 | 7% PEG 3350, 20% Trehalose, 10mM CdCl2, 100mM HEPES, di-mu-iodobis(ethylenediamine)diplatinum nitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 7 (%) | |
2 | 1 | reservoir | trehalose | 20 (%) | |
3 | 1 | reservoir | 10 (mM) | ||
4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |