Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1MMT

Crystal structure of ternary complex of the catalytic domain of human phenylalanine hydroxylase (Fe(II)) complexed with tetrahydrobiopterin and norleucine

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE BM1A
Synchrotron siteESRF
BeamlineBM1A
Temperature [K]110
Detector technologyIMAGE PLATE
Collection date2002-02-02
DetectorMARRESEARCH
Wavelength(s)0.800
Spacegroup nameC 2 2 21
Unit cell lengths65.140, 106.638, 123.611
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000

*

- 2.000
Rwork0.213
R-free0.24300
Structure solution methodphases adopted from 1KW0
Starting model (for MR)1kw0
RMSD bond length0.006
RMSD bond angle21.300

*

Data reduction softwareDENZO
Data scaling softwareSCALA
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.110
High resolution limit [Å]2.0002.000
Rmerge0.0550.275
Number of reflections285524197

*

<I/σ(I)>7.72.2
Completeness [%]97.599.1
Redundancy3.53.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.827720% PEG 2000, 12% ethylene glycol, 0.12M Na-Hepes, 10mM BH4, 30mM Na-dithionite, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirPEG200020 (%(w/v))
21reservoirethylene glycol12 (%(v/v))
31reservoirsodium HEPES0.12 (M)pH6.8
41reservoir10 (mM)
51reservoirsodium dithionite30 (mM)
61dropprotein7-8 (mg/ml)

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon