1MHP
Crystal structure of a chimeric alpha1 integrin I-domain in complex with the Fab fragment of a humanized neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 65 |
Unit cell lengths | 255.090, 255.090, 38.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 500.000 * - 2.800 |
R-factor | 0.222 |
Rwork | 0.213 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.430 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 * | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.083 | 0.309 |
Number of reflections | 35275 | |
<I/σ(I)> | 11.92 | 2.29 |
Completeness [%] | 96.6 | 87.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | 20 * | PEG 1500, Tris, NaCl, beta-mercaptoethanol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | 1 (mM) | ||
5 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
6 | 1 | reservoir | PEG1500 | 20-30 (%(w/v)) |