1M6D
Crystal structure of human cathepsin F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.250, 103.260, 58.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.22000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f2a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.340 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 * | 0.431 * |
| Total number of observations | 591413 * | |
| Number of reflections | 43466 | |
| <I/σ(I)> | 32 | 3.5 |
| Completeness [%] | 93.7 | 64.5 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 290 | magnesium acetate, sodium cacodylate, pEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6. |
| 3 | 1 | reservoir | PEG8000 | 25 (%(w/v)) | |
| 4 | 1 | drop | cathepsin | 3 (mg/ml) | |
| 5 | 1 | drop | sodium acetate | 50 (mM) | pH4.0 |
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | drop | 150 (mM) |
