1M5V
Transition State Stabilization by a Catalytic RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2001-11-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 258.648, 44.258, 102.174 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.400 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HP6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 43840 | |
| <I/σ(I)> | 24.7 | 3.1 |
| Completeness [%] | 97.7 | 81.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 300 | MPD, ammonium chloride, calcium chloride, pH 5.0, VAPOR DIFFUSION, SITTING DROP at 300K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CaCl2 | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | CaCl2 | ||
| 5 | 1 | 2 | NH4Cl | ||
| 6 | 1 | 2 | MPD |
